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Golden Gate Cloning
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7008510Golden Gate Cloninghttps://www.gandhi.com.mx/golden-gate-cloning-9781071642207/phttps://gandhi.vtexassets.com/arquivos/ids/6549110/image.jpg?v=63863664973317000034653849MXNSpringer USInStock/Ebooks/<p>This volume provides an overview of the latest protocols that cover the full range of <em>in silico</em>, <em>in vitro</em>, and <em>in vivo</em> approaches to help researchers plan, design, and perform Golden Gate cloning experiments. The chapters in this book cover topics such as Golden Gate cloning of standardized parts; modular DNA construct design for high-throughput Golden Gate Assembly; Golden Gate cloning of Synthetic CRISPR RNA spacer sequences; Golden Gate cloning in Actinobacteria; and YeastFab cloning of toxic genes and protein expression optimization in yeast. Written in the highly successful <em>Methods in Molecular Biology</em> series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.</p><p>Cutting-edge and thorough, <em>Golden Gate Cloning: Methods and Protocols</em> is a valuable resource for all researchers interested in learning more about this important and developing field.</p><p>Chapter 3 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.</p>...6680112Golden Gate Cloning34653849https://www.gandhi.com.mx/golden-gate-cloning-9781071642207/phttps://gandhi.vtexassets.com/arquivos/ids/6549110/image.jpg?v=638636649733170000InStockMXN99999DIEbook20249781071642207_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_<p>This volume provides an overview of the latest protocols that cover the full range of <em>in silico</em>, <em>in vitro</em>, and <em>in vivo</em> approaches to help researchers plan, design, and perform Golden Gate cloning experiments. The chapters in this book cover topics such as Golden Gate cloning of standardized parts; modular DNA construct design for high-throughput Golden Gate Assembly; Golden Gate cloning of Synthetic CRISPR RNA spacer sequences; Golden Gate cloning in Actinobacteria; and YeastFab cloning of toxic genes and protein expression optimization in yeast. Written in the highly successful <em>Methods in Molecular Biology</em> series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.</p><p>Cutting-edge and thorough, <em>Golden Gate Cloning: Methods and Protocols</em> is a valuable resource for all researchers interested in learning more about this important and developing field.</p><p>Chapter 3 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.</p>...9781071642207_Springer USlibro_electonico_9781071642207_9781071642207InglésMéxico2024-11-04T00:00:00+00:002024-10-03T00:00:00+00:00Springer US